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Annual Review of Genetics

Volume 57, 2023

Leveraging Single-Cell Populations to Uncover the Genetic Basis of Complex Traits

Abstract

The ease and throughput of single-cell genomics have steadily improved, and its current trajectory suggests that surveying single-cell populations will become routine. We discuss the merger of quantitative genetics with single-cell genomics and emphasize how this synergizes with advantages intrinsic to plants. Single-cell population genomics provides increased detection resolution when mapping variants that control molecular traits, including gene expression or chromatin accessibility. Additionally, single-cell population genomics reveals the cell types in which variants act and, when combined with organism-level phenotype measurements, unveils which cellular contexts impact higher-order traits. Emerging technologies, notably multiomics, can facilitate the measurement of both genetic changes and genomic traits in single cells, enabling single-cell genetic experiments. The implementation of single-cell genetics will advance the investigation of the genetic architecture of complex molecular traits and provide new experimental paradigms to study eukaryotic genetics.

Keyword(s): chromatin accessibility, gene expression, mutants, population genetics, quantitative genetics, single-cell genomics
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/content/journals/10.1146/annurev-genet-022123-110824
2023-11-27
2024-04-27
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Leveraging Single-Cell Populations to Uncover the Genetic Basis of Complex Traits
Annual Review of Genetics 57, 297 (2023); https://doi.org/10.1146/annurev-genet-022123-110824
/content/journals/10.1146/annurev-genet-022123-110824
/content/journals/10.1146/annurev-genet-022123-110824
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